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RT-PCR analysis of MUC5AC mRNA expression from A549 cells. Total RNA was extracted from confluent cultures and analyzed for the presence of MUC5AC and GAPDH transcripts by RT-PCR. The amplified products were run on 1% agarose-ethidium bromide gels. The results were expressed as means ± SE of six different experiments. * p < 0.01 versus control, † < 0.05 versus NOR-1 stimulated cells.
