Table 4.
Complete assignment of the NMR spectra of the mono-O-acetylated polysaccharide
| NRT36 Mono-O-Acetyl ~50°C | H-1 | H-2 | H-3 | H-4 | H-5 | H-6, H-6' |
| C-1 | C-2 | C-3 | C-4 | C-5 | C-6 | |
| A: α-D-GlcA | 5.15 | 3.63 | 3.67 | 3.51 | 3.95 | - |
| 101.9 | 72.7 | 73.8 | 72.7 | 74.1 | 176.7 | |
| B: α-D-GlcNAc | 4.99 (5.01) | 3.91 (4.05) | 3.99 (5.27) | 3.63 (3.87) | 4.14 | 4.09, 3.71 |
| 94.9 (94.1) | 53.9 (52.6) | 71.6 (74.3) | 80.3 | 68.6 | 68.4 | |
| C: β-L-Rha | 5.04 | 5.53 | 3.80 | 3.48 | 3.49 | 1.36 |
| 99.9 | 69.9 | 76.3 | 71.6 | 73.2 | 17.6 | |
| D: β-D-GlcNAc | 4.39 | 3.77 | 3.66 | 3.69 | 3.43 | 3.90, 3.88 |
| 102.1 | 56.3 | 74.7 | 77.8 | 75.1 | 61.7 | |
| a.) Shifts enclosed in parentheses are those of the 3-O-acetylated B residue in the native polysaccharide. | ||||||
| assignment of NAc's, OAc's | NH | C = O | CH3 | |||
| B: α-D-GlcNAc | 7.79 | 174.9 | 22.93; 2.03 | |||
| D: β-D-GlcNAc | 8.09 | 175.2 | 23.19; 2.08 | |||
| C: β-L-Rha OAc | 173.9 | 21.26; 2.21 | ||||