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. 1990 Sep;56(9):2704–2710. doi: 10.1128/aem.56.9.2704-2710.1990

Risk Assessment Studies: Detailed Host Range Testing of Wild-Type Cabbage Moth, Mamestra brassicae (Lepidoptera: Noctuidae), Nuclear Polyhedrosis Virus

Cathy J Doyle 1,*, Mark L Hirst 1, Jenny S Cory 1, Philip F Entwistle 1
PMCID: PMC184831  PMID: 16348279

Abstract

The host range of a multiply enveloped nuclear polyhedrosis virus (NPV) (Baculoviridae) isolated from the cabbage moth, Mamestra brassicae (Lepidoptera: Noctuidae), was determined by challenging a wide range of insect species with high (106 polyhedral inclusion bodies) and low (103 polyhedral inclusion bodies) doses of the virus. The identity of the progeny virus was confirmed by dot blotting. Analysis of 50% lethal dose was carried out on selected species, and the progeny virus was identified by using restriction enzyme analysis and Southern blotting. Other than the Lepidoptera, none of the species tested was susceptible to M. brassicae NPV. Within the Lepidoptera, M. brassicae NPV was infective to members of four families (Noctuidae, Geometridae, Yponomeutidae, and Nymphalidae). Of 66 lepidopterous species tested, M. brassicae NPV was cross-infective to 32 of them; however, 91% of the susceptible species were in the Noctuidae. The relevance of host range data in risk assessment studies is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown D. A., Evans H. F., Allen C. J., Kelly D. C. Biological and biochemical investigations on five European isolates of Mamestra brassica nuclear polyhedrosis virus. Arch Virol. 1981;69(3-4):209–217. doi: 10.1007/BF01317336. [DOI] [PubMed] [Google Scholar]
  2. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  3. Evans H. F. The influence of larval maturation on responses of Mamestra brassicae L. (Lepidoptera: Noctuidae) to nuclear polyhedrosis virus infection. Arch Virol. 1983;75(3):163–170. doi: 10.1007/BF01315270. [DOI] [PubMed] [Google Scholar]
  4. Kafatos F. C., Jones C. W., Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 1979 Nov 24;7(6):1541–1552. doi: 10.1093/nar/7.6.1541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kelly D. C., Brown D. A. Biochemical and biophysical properties of a Mamestra brassicae multiple enveloped nuclear polyhedrosis virus. Arch Virol. 1980;66(2):133–141. doi: 10.1007/BF01314981. [DOI] [PubMed] [Google Scholar]
  6. Oakey R., Cameron I. R., Davis B., Davis E., Possee R. D. Analysis of transcription initiation in the Panolis flammea nuclear polyhedrosis virus polyhedrin gene. J Gen Virol. 1989 Mar;70(Pt 3):769–775. doi: 10.1099/0022-1317-70-3-769. [DOI] [PubMed] [Google Scholar]
  7. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]

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