Figure 5.

Reorganization of endogenous α-internexin in neuronal precursor cells expressing Tax. Tax expression vector was transfected into neuronal precursor cells, and 48 hr posttransfection cells were fixed, stained with anti-Tax antibodies, and visualized by confocal microscopy. The endogenous a-internexin was identified by the staining of the untransfected cells with the anti-α-internexin antibody. For the colocalization of Tax and endogenous α-internexin, the cells transfected with Tax were stained with anti-Tax and anti-α-internexin antibodies. (A) Tax alone stained with anti-Tax antibody followed by secondary antibodies conjugated with rhodamine. (B) Endogenous α-internexin stained with anti-α-internexin antibody followed by secondary antibodies conjugated with FITC (filamentous form with green fluorescence). (C–K) Various cells expressing both endogenous α-internexin and Tax. (C, F, and I) Rhodamine staining because of Tax. (D, G, and J) Green fluorescence because of endogenous α-internexin. (E, H, and K) Colocalization of endogenous α-internexin and Tax in the same cell as shown in C, F, and I and D, G, and J as visualized by both colors.