Figure 1.

(A) Rho fluorescence histograms of sorted WGA⁺/Lin⁻ cells derived from mobilized blood (solid line) or steady-state bone marrow (shaded line). The Rho⁻ cell fraction contained 0.9 ± 0.3% and 6.5 ± 1.0% of all WGA⁺/Lin⁻ cells from blood and bone marrow, respectively. (B) Recipient mice survival for at least 4 weeks after lethal irradiation (8.5 Gy) and transplantation of cells from the Rho⁻, Rho⁺, and Rho⁺⁺ fractions of WGA⁺/Lin⁻ cells derived from mobilized blood (solid lines). In three different experiments a total of 18–28 mice was transplanted for each cell dose. The survival of mice transplanted with steady-state bone-marrow-derived WGA⁺/Lin⁻/Rho⁻ cells (dashed line) is derived from previous studies (17).