Figure 3.

Peripheral blood chimerism of individual mice at various times after cotransplantation of bone-marrow- and blood-derived cells. Female recipient mice were transplanted with bone-marrow-derived cells from a male donor and blood-derived cells from a female donor (group A, dashed lines) or with bone-marrow-derived cells from a female donor and blood-derived cells from a male donor (group B, solid lines). Each line represents the analysis of an individual animal. The mean percentage of male cells increased from 41 to 75% in group A and decreased from 65 to 24% in group B. Because the blood graft (15 × 10⁴ unseparated cells) and bone marrow graft (4 × 10⁴ unseparated cells) contained WGA⁺/Lin⁻/Rho⁻ stem cells at a ratio of 3:2 (see Table 1), the results indicate an equal STRA of blood- and bone-marrow-derived stem cells at day 20, but a ±5-fold reduced LTRA of blood-derived stem cells at day 110.