Table 1.
CFCs in the Rho− and Rho+/++ fractions of WGA+/Lin− cells derived from mobilized blood or steady-state bone marrow
| Mobilized blood
|
Bone marrow
|
|||
|---|---|---|---|---|
| Rho− | Rho+/++ | Rho− | Rho+/++ | |
| CFC/750 sorted cells, no. | 3 ± 4 | 25 ± 18 | 1 ± 1 | 36 ± 20 |
| Graft composition | ||||
| Cells, no. | 74 ± 18 | 6,600 ± 2,200 | 67 ± 7 | 910 ± 110 |
| CFCs, no. | 0.3 ± 0.3 | 260 ± 170 | 0.1 ± 0.1 | 47 ± 20 |
Absolute numbers in the grafts represent the analysis of a blood cell graft of 5 × 104 cells and a bone marrow graft of 2 × 104 cells, i.e., the minimal graft size that was required for 90% survival of transplanted lethally irradiated recipients. The absolute numbers of CFCs in unseparated blood and bone marrow grafts of similar size were 211 ± 45 and 40 ± 14 cells, respectively. Results are expressed as the mean ± SD of at least three experiments involving 30 mice.