Table 1.
Gene complementation
| Strain | Growth in arabinose
|
|
|---|---|---|
| 0% | 0.04% | |
| ΔtcpP | — | |
| ΔtcpP (pBAD-P) | — | ++ |
| ΔtcpP (PBAD-P:bla) | — | ++ |
| ΔtcpP (pBAD-T) | — | ++ |
| ΔtcpP (pBAD-R) | — | — |
| ΔtoxR | — | |
| ΔtoxR (pBAD-R) | — | ++ |
| ΔtoxR (pBAD-T) | — | ++ |
| ΔtoxR (pBAD-P) | — | + |
| ΔtoxT | — | |
| ΔtoxT (pBAD-T) | — | ++ |
| ΔtoxT (pBAD-R) | — | — |
| ΔtoxT (pBAD-P) | — | — |
V. cholerae strains with chromosomal deletions in tcpP, toxR, or toxT carrying plasmids with these genes under an arabinose-inducible promoter were grown overnight in LB medium at 30°C without or with 0.04% arabinose, followed by transduction. A + indicates at least 50 Kan-resistant colonies, whereas a ++ indicates a confluent lawn of Kan-resistant colonies in 25 μl of plated culture.