Table 3.
Expression level of the signal peptide-EPO fusion constructs
| Plasmid | Signal peptide | Second codon | Expression levela(μg/ml) | Relative level of expressionb |
|---|---|---|---|---|
| pK7pelBss-EPO | pelB | AAA | 569 ± 34 | 10.3 |
| pK7ompAss-EPO | ompA | AAA | 588 ± 28 | 10.7 |
| pK7phoAss-EPO | phoA | AAA | 515 ± 31 | 9.4 |
| pK7malEss-EPO | malE | AAA | 524 ± 13 | 9.5 |
| pK7ompCss-EPO | ompC | AAA | 522 ± 24 | 9.5 |
| pK7ompTss-EPO | ompT | CGG | 107 ± 17 | 1.9 |
| pK7heposs-EPO | heposs | GGG | 101 ± 13 | 1.8 |
aData from three independent experiments.
bExpression level as compared with the expression of the wild-type EPO (EPO expression level = 1; EPO expression level = 55 μg/ml)