Table 4.
Cell-free expression of the ompAss fused genes
| Protein | Wild-type (μg/ml) | ompA fusion (μg/ml) | Relative level of expressiona | GenBank ID/references | Molecular weight of protein (kDa) | Origin |
|---|---|---|---|---|---|---|
| IL-2 | 91 | 545 | 6.0 | S77834 | 15.5 | Homo sapiens |
| TNF-a | 226 | 513 | 2.3 | X01394 | 17.5 | Homo sapiens |
| DsRed2b | 98 | 665 | 6.8 | — | 25.9 | Synthetic gene |
| BMP2 | 128 | 431 | 3.4 | M22489 | 13.0 | Homo sapiens |
| UK | 83 | 387 | 4.7 | NM_008873 | 30.5 | Mus musculus |
| VEGF | n.d. | 489 | — | X62568 | 22.3 | Homo sapiens |
| IFN-γ | 45 | 460 | 10.2 | K00083 | 16.0 | Mus musculus |
| ϖ-TACc | 125 | 487 | 3.9 | AAK25105 | 47.8 | Caulobacter crescentus |
| ϖ-TAMl | 206 | 537 | 2.6 | BAB48961 | 48.2 | Mesorhozobium loti |
| ϖ-TATr | 173 | 436 | 2.5 | AAL44116 | 49.0 | Agrobacterium tumefaciens |
| ϖ-TAVf | 237 | 539 | 2.3 | (32) | 50.3 | Vibrio fluvialis |
| ϖ-TAXc | 198 | 529 | 2.7 | AAM41635 | 48.4 | Xanthomonas axonopodis |
| smTG | 11 | 358 | 32.5 | AAS68222 | 45.7 | Streptomyces mobaraensis |
| oleV | 35 | 645 | 18.4 | AAD55451 | 53.1 | Streptomyces antibioticus |
| oleW | 26 | 689 | 26.5 | AAD55450 | 35.8 | Streptomyces antibioticus |
| urdR | n.d. | 702 | — | AAF72551 | 26.8 | Streptomyces fradiae |
aThe relative expression level was determined by dividing the amount of the ompA fused protein by the expressed wild-type protein.
bPlasmid pDsRed2 is obtained from Clontech.
n.d., not determined.