Figure 2.

Effect of arsenicals on incorporation of selenium into selenoproteins in DMEM containing 10% serum. HaCat cells were cultivated in the presence of ⁷⁵Se–selenite (10 nM) and treated with sodium arsenite, sodium arsenate, MMA^III, or DMA^III at the concentrations indicated. Cells were harvested after 24-hr incubation with arsenicals and radioisotope selenium. Twenty-five micrograms of protein from crude cell extract were separated by 15% SDS-PAGE. Selenoproteins were identified by autoradiography using standard protein markers (molecular weight indicated at left of autoradiogram) based on Coommassie-stained gels before exposure.