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. 2006 Dec 19;115(3):346–353. doi: 10.1289/ehp.9440

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Treatment of HaCat with trivalent arsenicals results in increased expression of TrxR1 (A) and decreased expression of cGpx (B) when cells are treated in medium containing serum. Real-time RT-PCR analysis was used to determine the relative mRNA levels of transcripts encoding TrxR1 and cGpx. Fold induction is plotted versus control cultures (no arsenical addition). β-Actin levels were used as an internal control for quantitative RT-PCR analysis. See ”Materials and Methods” for details. Error bars represent mean ± SD for at least three independent RNA samples isolated from independent cultures.