Table 2.
Expression of MMPs by Cell Lines Established from Vulvar Carcinomas
| Cell line | Age | Type of lesion | Tumor grade | TNM at diagnosis | HPV status | p53 gene status | mRNA levels | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| MMP-13 | MMP-1 | MMP-7 | MT1-MMP | MMP-9 | |||||||
| UM-SCV-1A | 62 | primary | II–III | T3N2M1 | − | WT | 1.3 | − | 5.6 | 0.7 | − |
| UM-SCV-1B | 62 | metastasis | III | T3N2M1 | − | WT | 4.0 | 3.0 | 4.5 | 5.0 | − |
| UM-SCV-2 | 86 | recurrent | III | T3N1M0 | − | + | 4.3 | 3.0 | 0.9 | 1.0 | − |
| UM-SCV-3 | 66 | primary | II | T2N0M0 | − | + | − | − | − | 0.7 | − |
| UM-SCV-4 | 41 | primary | I | T2N2M0 | − | + | − | − | − | 0.1 | − |
| UM-SCV-5 | 60 | recurrent | II | N.A. | − | + | 1.0 | − | 0.4 | 0.2 | 0.3 |
| UM-SCV-6 | 43 | recurrent | II | T1N1M0 | HPV16 | WT | 10 | − | − | 0.5 | − |
| UM-SCV-7 | 77 | primary | II–III | T2N2M0 | − | + | 0.2 | 1.0 | 0.9 | 0.5 | − |
| UT-SCV-1 | 70 | metastasis | I | T1N0M0 | − | + | 0.3 | − | − | 0.1 | − |
| UT-SCV-2 | 74 | recurrent | II | T1N0M0 | − | WT | 15 | − | 0.7 | 0.1 | − |
| UT-SCV-3 | 73 | primary | II | T3N2M0 | N.D. | N.D. | 6.8 | 12 | 0.2 | 0.1 | − |
mRNA levels were determined by Northern blot hybridizations, quantitated by densitometry, and corrected for the levels of GAPDH mRNA. UT-SCV-1 cell line is derived from patient # 1, UT-SCV-2 cell line from patient # 2 and UT-SCV-3 cell line from patient # 3.
N.D., not determined; N.A., not available; WT, wild-type p53 gene; +, mutated.