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. 1999 Feb;154(2):553–566. doi: 10.1016/s0002-9440(10)65301-1

Figure 4.

Figure 4.

Appearance of TRAP-positive cells in the femur of a young wild-type mouse (A), a young op/op mouse (B), an aged op/op mouse (C), or a GM-CSF- (D), an IL-3- (E), or a GM-CSF- plus IL-3-treated (F) young op/op mouse. A and C to F: TRAP-positive cells are seen on the endosteal surfaces of the femur diaphysis in the young wild-type mouse, the aged op/op mouse, and the treated young op/op mouse. Arrowheads show TRAP-positive cells, and arrows indicate TRAP-positive resorption surfaces where no osteoclasts are detected. Cells in the center of a black square are shown in the inset at a higher magnification. A: There are numerous TRAP-positive osteoclasts in a young wild-type mouse. Inset: A high magnification of a multinucleated osteoclast. B: TRAP-positive cells are absent in the osteopetrotic femur bone of the young op/op mouse. C to F: Increased numbers of osteoclasts are seen in an aged op/op mouse (C) and a GM-CSF- (D), an IL-3- (E), and a GM-CSF- plus IL-3-treated (F) young op/op mouse. Inset: Mononuclear TRAP-positive cells in the aged (C) and GM-CSF-treated young (D) op/op mouse and a multinucleated osteoclast in GM-CSF- plus IL-3-treated young op/op mouse (F). TRAP staining; magnification, ×66 and ×100 (inset).