Figure 2.

Assessment of transgene mRNA levels in retinal RNA by RT-PCR. Total RNA was extracted from the retinas of rho/PDGF-A mice, and RT-PCR was done for PDGF-A transgene mRNA and S16 ribosomal protein mRNA. The size of the band amplified with transgene primers was compatible with the 538 bp predicted for amplification from mRNA and was absent (as was the band for S16) when RT was excluded from the reaction. A: In adult mice, a strong signal (lines 1–3), an intermediate signal (line 5), and a weak signal for (line 4) were seen. B: A weak signal was seen at P3 and increased to a steady-state level between P10 and P14 (lines 2 and 3).