Figure 6.

rho/PDGF-A transgenic mice do not develop hyperoxia-induced retinal NV. At P7, transgene-positive and littermate control mice were placed in 75% oxygen for 5 days followed by room air for 5 days and then sacrificed. Retinal frozen sections were stained with GSA. A: A transgene-negative mouse shows an area of retinal vascular nonperfusion (delimited by arrows) and large clumps of NV on the surface of the retina (arrowheads). B: A transgene-positive mouse shows no areas of vascular nonperfusion and no NV. C: Image analysis was performed to measure the area of NV, as described in Materials and Methods, on retinas from 10 transgene-negative and 10 transgene-positive mice. There was a dramatic difference with substantial NV in transgene-negative mice and no NV identified in any of the transgene-positive mice. *P < 0.0001 by two-tailed t-test.