Skip to main content
. 2000 Feb;156(2):477–487. doi: 10.1016/S0002-9440(10)64752-9

Figure 9.

Figure 9.

Retinal expression of mRNA for endothelial survival factors in neonatal rho/PDGF-A transgene-positive and -negative mice during and after hyperoxia. rho/PDGF-A+/− mice were crossed with wild-type mice, and resultant litters were placed in 75% oxygen at P7 and removed to room air at P12. Mice were sacrificed at P7, before placement in hyperoxia or 4 hours after placement in hyperoxia, P10 (after 3 days of hyperoxia), P12 (after 5 days of hyperoxia and 1 hour of hypoxia), P14 (2 days after removal from hyperoxia to the relative hypoxia of room air), and P17 (after 5 days of hypoxia when there is severe NV). Total retinal RNA was isolated and 10 μg were used for each lane for Northern blots. The blots were hybridized with a VEGF cDNA probe and, after exposure, were stripped and hybridized with a probe for IGF-I. Blots were successively stripped and rehybridized with probes for FGF2, PDGF-A, and 18S. At P12 and P14, during the period when there was an endothelial rescue effect in transgene-positive mice as judged by the decreased vascular nonperfusion, FGF2 mRNA expression was increased in transgene-positive mice compared with transgene-negative mice, although this was not true for the other survival factors.