Figure 1.

Extraction of proteins. Fixed, stained, microscopic tissue cells of interest are selected using LCM and transferred onto the area of the polymer surface activated by the laser beam. The captured cells are exposed to concentrated extraction buffer in a microvolume. The solubilized proteins are diluted in the immunoassay buffer and incubated with a solid-phase capture antibody specific for the analyte (Materials and Methods). Chemiluminescent detection is conducted with a labeled secondary antibody through a sandwich binding step.