Immunohistochemical staining of CA XII in the segments of the human gut. The epithelia of the duodenum (A), jejunum (B), and ileum (C) give no signal for CA XII, whereas all segments of the large intestine, ie, the cecum (D), ascending colon (E), and rectum (F), demonstrate a strong positive reaction. The intensity of staining for CA XII was highest in the epithelial cells of the surface epithelial cuff and decreased toward the base of the glandular crypts. The subcellular distribution of CA XII in the epithelial cells is confined to the basolateral plasma membrane, as seen in the confocal laser scanning microscopy image (G). The arrows mark the apical brush border. Specimens were fixed with Carnoy’s fluid. Original magnifications: A–F, ×400; G, ×630.