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. 2000 Feb;156(2):685–695. doi: 10.1016/S0002-9440(10)64772-4

Figure 4.

Figure 4.

Indirect immunofluorescence staining with autoantibodies immunoadsorbed with the soluble ectodomain. Pemphigoid and LAD sera, and a pemphigus vulgaris serum as a control, were immunoadsorbed with the soluble ectodomain bound to a solid phase and used for indirect immunofluorescence staining of NaCl-split normal human skin. IgG autoantibodies from a pemphigoid serum (A) and IgA autoantibodies from a LAD serum (B) stained the blister roof. C: The IgG autoantibodies from the pemphigoid serum used in A showed a negative reaction on collagen XVII-deficient skin from a GABEB patient. D: From pemphigus vulgaris serum, no antibodies reactive with human skin were immunoadsorbed with the 120-kd ectodomain. In all panels, the asterisk indicates the blister cavity.