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. 2000 Feb;156(2):685–695. doi: 10.1016/S0002-9440(10)64772-4

Figure 6.

Figure 6.

Immunoblotting of IgG and IgA autoantibodies with recombinant collagen XVII fragments. Recombinant fragments spanning the Ecto2 (A), NC16a (B), and Col15 domains (C) were reacted with different bullous pemphigoid sera and with a LAD serum in immunoblots. In each blot, C designates the lane reacted with a control antibody raised against the recombinant fragment. Lanes 1–4 show reactions with different pemphigoid sera, and LAD indicates a reaction with a LAD serum. On the left of the panels, migration positions of molecular mass markers are indicated. From top to bottom: 47, 34, and 28 kd.