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. 2000 Jun;156(6):2033–2043. doi: 10.1016/S0002-9440(10)65075-4

Figure 1.

Figure 1.

Development of colonies derived from porcine nonparenchymal epithelial cell clusters. The cells within the same field marked by a needle are shown. A: Day 1 (28 hours after plating). The photograph within the square is at a higher magnification. NPECs are observed as clusters. B: Day 2 (50 hours). NPECs spread to about twice in area. C: Day 3 (81 hours). NPEC clusters begin to proliferate. D: Day 4 (101 hours). NPEC clusters begin to scatter. E: Day 4 (130 hours). F: Day 5 (141 hours). G: Day 7 (166 hours). H: Day 8 (197 hours). Arrows indicate a duct-like structure. I: Day 9 (220 hours). J: Day 10 (244 hours). K: Day 12 (292 hours). L: Day 15 (360 hours). Arrows indicate several duct-like structures. The cells, containing dark cytoplasm that surrounded by the duct-like structures, adopt a cobblestone-like morphology. The star indicates the position highly magnified in Figure 2 ▶ . Original magnifications, ×200 (A−C; scale bar, C, 50 μm) and ×100 (D−L; scale bar, L, 100 μm).