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Kinase activities associated with cdk2 (a), cyclin A (b), or cyclin E (c) in surgically resected tissues. Lysates (200 μg) were immunoprecipitated with anti-cdk2, anti-cyclin A, or anti-cyclin E antibodies. The immunocomplexes were assayed for kinase activity using glutathione S-transferase-retinoblastoma fusion protein as a substrate. N, normal tissue; T, tumor tissue.
