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Immunohistochemical staining of MKK4 kinase in human gastric adenocarcinoma sections. Anti-MKK4 protein antibody was used for immunohistochemical staining in human gastric cancer tissues by avidin-biotin-peroxidase complex methods as described in Methods. A: Positive MKK4 kinase immunoreactivity existed in the cytoplasm and nucleus. B: Absence of MKK4 immunoreactivity was observed in the presence of MKK4 blocking peptide in the staining reaction (original magnification, ×400).
