Table 3.
Genes Identified by GeneCalling
| Confirmed gene* | Accesion no.† | GeneCalling ratio‡ | TaqMan ratio§ |
|---|---|---|---|
| Hormones/growth factors | |||
| Placental growth factor | X54936 | 6 | 5 |
| Transforming growth factor beta | X02812 | 11 | 2 |
| Stanniocalcin precursor | U25997 | 14 | 8 |
| FGF-16 | AB009391 | 4 | 1 |
| Angiopoetin-2 Hlog | AI079861 | 0.1 | 0.1 |
| Connective tissue growth factor | U14750 | 0.1 | 0.2 |
| Cell cycle/apoptosis | |||
| Human replication factor C | M87338 | 5 | 23 |
| ALG-2 interacting protein | sim to AJ005073 | 5 | 2 |
| p120 proliferation associated antigen | X55504 | 4 | 1 |
| CDEBP (amyloid precursor-like protein 2) | Z22572 | 4 | 1 |
| Bcl-2 related protein A1 | L19597 | 4 | 1 |
| ABC50 ATP binding cassette protein | AF027302 | 2 | ND |
| cdc28/cdc2 associated protein CLK | L29219 | <0.1 | 0.3 |
| Polo-like kinase | U01038 | <0.1 | <0.1 |
| p53 | AA143745 | 0.1 | 1 |
| CDK4 inhibitor (p16-INK4) | L27211 | 5 | ND |
| DNA binding protein transcription factors histones/repair | |||
| KIAA0192/TRAP230 | AF117755 | 3 | 1 |
| Mel-18 hlog | AA477595 | 4 | ND |
| OS-9 | AB002806 | 2 | 1 |
| DNA nucleotide exotransferase | AA744855 | 2 | ND |
| Histone HUMGS00579 | M37583 | 0.2 | 0.3 |
| oriP binding protein (OBP-2) | L29606 | <0.1 | ND |
| RNA synthesis/ribosomes | |||
| Clone 23689 | AF035280 | 8 | 8 |
| Ribosomal protein L37a | L22154 | 4 | ND |
| Chemokines | |||
| Interleukin-8 | M28130 | 0.3 | <0.1 |
| GRO1α (melanoma growth-stimulating activity) | X57019 | <0.1 | <0.1 |
| Tyrosine kinase receptors | |||
| eck | NM_004431 | 3 | 3 |
| axl | P30530 | 0.2 | <0.1 |
| Serine/threonine kinases | |||
| Serum inducible kinase | O60679 | 7 | 2 |
| Branched chain alpha keto acid dehydrogenase kinase | AF026548 | 4 | 1 |
| Thymidylate kinase | L16991 | 0.5 | 0.3 |
| Other receptors/integral membrane glycoproteins | |||
| OX40 | S76792 | 18 | 18 |
| CXCR4 | AF052572 | 13 | 6 |
| Alpha 2 integrin | X17033 | 13 | 2 |
| Podocalyxin-like protein | U97519 | 12 | 2 |
| CD82 | D28137 | 12 | 4 |
| MUC18 | M29277 | 5 | 1 |
| MHC class I | M83191 | 3 | 3 |
| D14343 | |||
| M20022 | |||
| gp130 | M57230 | 3 | 1 |
| T-cell receptor beta 2 | X01411 | 3 | 2 |
| MHC class II | L18885 | 2 | ND |
| Protein zero related protein | R60084 | 6 | 6 |
| Proteases/protease inhibitors | |||
| Tissue factor pathway inhibitor-2 | L27624 | 9 | 7 |
| Aggrecanase (ADAMTS4) | NM_005099 | 18 | 2 |
| Type IV collagenase (MMP9) | J05070 | 6 | 177 |
*Gene identified and confirmed by GeneCalling.
†GeneBank accession number of gene from which cDNA fragment was identified.
‡Refers to the median value of ratios for 24 hours/4 hours for the different cDNA fragments identified for a specific gene.
§TaqMan Ratio refers to the ratio of mRNA at 24 hours versus 4 hours as determined by Taqman (see Materials and Methods).
ND, not determined.