Table 4.
Response of Modulated Genes to PPARγ Ligand 15d-PDJ2
| Confirmed gene* | Accesion no.† | TaqMan ratio‡ | TaqMan ratio in 15d-PDJ2-treated cells§ |
|---|---|---|---|
| Hormones/growth factors | |||
| Placental growth factor | X54936 | 5 | 0.4 |
| FGF-16 | AB009391 | 1 | 1 |
| Stanniocalcin precursor | U25997 | 8 | 3 |
| Cell cycle/apoptosis | |||
| Bcl-2 related protein A1 | L19597 | 1 | 1 |
| Polo-like kinase | U01038 | <0.1 | 15.5 |
| RNA synthesis/ribosomes | |||
| Clone 23689 | AF035280 | 8 | 0.3 |
| Chemokines | |||
| Interleukin-8 | M28130 | <0.1 | 14.5 |
| Tyrosine kinase receptors | |||
| eck | NM_004431.1 | 3 | 2.3 |
| axl | P30530 | <0.1 | 15 |
| Other receptors/integral membrane glycoproteins | |||
| OX40 | S76792 | 18 | 0.7 |
| Podocalyxin-like protein | U97519 | 2 | 1 |
| Proteases/protease inhibitors | |||
| Tissue factor pathway inhibitor-2 | L27624 | 7 | 1.3 |
| Type IV collagenase (MMP9) | J05070 | 177 | <0.1 |
| Plasminogen activator inhibitor-2 | M31551 | <0.1 | 10.2 |
| KIAA0188 | d80010 | 2 | 0.5 |
| Transporters/channels | |||
| Glycoprotein-associated amino acid transporter LAT1 | AJ130718 | 20 | 0.2 |
| White protein Hlog | AF038175 | 0.5 | 2 |
| Intermediate filaments | |||
| Keratin K7 | X03212 | 0.1 | 0.3 |
| Extracellular matrix | |||
| Laminin gamma 2 chain | U31201 | 49 | 4.9 |
| Nidogen-2 (Osteonidogen) | D86425 | 2 | 1 |
| Lipids and lipid turnover | |||
| Phospholipase A2 gamma | AF058921 | 21 | 0.8 |
A subset of genes identified as modulated in experiment were tested for response to PPARγ ligand 15d-PDJ2. Data are expressed as the ratio of expression observed at 24 hours/4 hours in collagen gel.
*Gene identified and confirmed by GeneCalling.
†GeneBank accession number of gene from which cDNA fragment was identified.
‡TaqMan ratio refers to the ratio of mRNA at 24 hours versus 4 hours.
§The ratio of mRNA at 24 hours versus 4 hours in the 15d-PDJ2-treated groups.