Table 3.
Sequences of primers used for quantitative real-time RT-PCR
| Gene | Accession no. | cDNA sequence (5′–3′) F, forward; R, reverse | Amplicon length (bases) | Corresponding cDNA sequence position |
|---|---|---|---|---|
| 18S rRNA | X56974 | F: TTCGAACGTCTGCCCTATCA | 49 | 1535–1555 |
| R: ATGGTAGGCACGGCGACTA | 1584–1566 | |||
| aP2 | NM_024406 | F: GCGTGGAATTCGATGAAATCA | 67 | 246–266 |
| R: CCCGCCATCTAGGGTTATGA | 313–294 | |||
| Osteocalcin | L24430 | F: CGGCCCTGAGTCTGACAAA | 208 | 1023–1041 |
| R: GCCGGAGTCTGTTCACTACCTT | 1231–1210 | |||
| Runx2/Cbfal | NM_009820 | F: GGGCACAAGTTCTATCTGGAAAA | 54 | 169–191 |
| R: CGGTGTCACTGCGCTGAA | 240–223 | |||
| Dlx5 | AF072453 | F: TGACAGGAGTGTTTGACAGAAGAGT | 64 | 184–208 |
| R: CGGGAACGGAGCTTGGA | 248–232 | |||
| α1(I)Collagen | NM_007742 | F: ACTGTCCCAACCCCCAAAG | 59 | 311–329 |
| R: CGTATTCTTCCGGGCAGAAA | 370–351 | |||
| PPAR-γ2 | U09138 | F: GCTGTTATGGGTGAAACTCTG | 351 | 34–54 |
| R: ATAAGGTGGAGATGCAGGTTC | 384–364 | |||
| TGF-β1 | NM_011577 | F: TACAGCAAGGTCCTTGCCCT | 62 | 1873−1892 |
| R: GCAGCACGGTGACGCC | 1935−1920 | |||
| TGF-β2 | BC011055 | F: CAACACCATAAATCCCGAAGC | 66 | 682–702 |
| R: GGTCAGTGGTTCCAGATCCTG | 748–728 | |||
| TGF-β3 | BC014690 | F: GCAACTAGCTATCTCAGGTCCCTT | 79 | 2221–2244 |
| R: CCAGGGAATACATGAGAGAACCA | 2300–2278 | |||
| TβR-1 | NM_009370 | F: AGCAGTGACTGCCATGCG | 67 | 2337–2354 |
| R: CAGGCTAAACGTCTCAACTGCA | 2404–2383 | |||
| TβR-2 | D32072 | F: CATGTGAGAAGAATAAAATACGAGAACA | 93 | 3615–3642 |
| R: AATGTGTAAGGGAAGTTGCCTATGT | 3708–3684 | |||
| BMP2 | AY050249 | F: AACTGGCTAGAATATTAAGCACTGCA | 71 | 6082–6107 |
| R: AGTGATTTCCTAACTGCCCAGG | 6153–6132 | |||
| BMP4 | BC052846 | F: TCAAGGGAGTGGAGATTGGG | 60 | 916–935 |
| R: GCCATCATGGCCAAAAGTG | 976–958 | |||
| BMPR-1 A | BC042611 | F: TGCATCAAGACTCCAATCCTGA | 83 | 2086–2107 |
| R: ACAGAAAGCACCACTTTATGGACA | 2169–2146 | |||
| BMPR-1B | BC065106 | F: GCTGGGCGCAGAATCCT | 73 | 1560–1576 |
| R: GGACTCTGACATTTTGGCAAGG | 1633–1612 | |||
| BMPRII | U78048 | F: TCCACCTGGGTCATCTCCA | 63 | 2975–2993 |
| R: CCCTGTCACTGCCATTGTTG | 3038–3019 | |||
| Smad1 | BC058693 | F: TCCGTCTCTTGCAAACTATCGA | 75 | 1809−1830 |
| R: TTCGTCAGGTCTCCATCCTGT | 1884−1864 | |||
| Smad2 | BC021342 | F: CCCTTCAGTGCGATGCTCA | 74 | 1504–1522 |
| R: GAATACTACGACGGAGGAGCTGTT | 1578–1555 | |||
| Smad3 | NM_016769 | F: CACGCAGAACGTGAACACC | 100 | 485–503 |
| R: GGCAGTAGATAACGTGAGGGA | 585–565 | |||
| Smad4 | BC046584 | F: ACAGAGAACATTGGATGGACGA | 69 | 662–683 |
| R: ACGGGCATAGATCACATGAGG | 731–711 | |||
| Smad5 | BC050001 | F: CAAGGGCCTTGCCTGCT | 76 | 3469–3483 |
| R: GTCCGAGACCTATGACATGAAGACT | 3545–3521 | |||
| Smad6 | BC047280 | F: TGGCTGGAGATCCTACTCAACA | 62 | 1753–1774 |
| R: GGACGCTGCGGCACAG | 1815−1800 | |||
| Smad7 | NM_008543 | F: GCCCCCCCTTCCTGCT | 63 | 3103–3119 |
| R: CCAGCCAAGGGATGGTACC | 3166–3148 |