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Steady-state kinetic parameters of trypanosomal TbTUT4 mutants with RNA.
| Protein | Km (μM) | ± |
kcat (min−1) |
± |
kcat/Km (min−1 M−1) |
[kcat/Km]mutant/ [kcat/Km]w.t. |
|---|---|---|---|---|---|---|
| TUT4 | 0.2 | 0.2 | 0.3 | 0.08 | 1.5×106 | 1 |
| R121A | 132.0 | 44.5 | 1.5 | 0.35 | 1.1×104 | 0.007 |
| R141A | 72.6 | 4.5 | 1.3 | 0.05 | 1.8×104 | 0.012 |
| S148A | 0.2 | 0.4 | 1.0 | 0.5 | 5.3 × 106 | 3.5 |
| S188A | 0.3 | 0.3 | 0.1 | 0.05 | 3.0 × 105 | 0.2 |
| R307A | 10.0 | 2.6 | 0.5 | 0.04 | 5×104 | 0.03 |
