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. Author manuscript; available in PMC: 2008 Feb 23.
Published in final edited form as: J Mol Biol. 2006 Dec 12;366(3):790–805. doi: 10.1016/j.jmb.2006.12.017

Figure 3.

Figure 3

EPR spectra at 120K and distance calculation for N76R1/A196R1. (a) Sum of spectra of the N76R1 and A196R1 single mutants without illumination (blue), spectrum of N76R1/A196R1 before illumination (black), and the spectrum of N76R1/A196R1 after illumination (red), superimposed. All spectra are normalized to the same spin number. Panels (b) and (c) show the distance distributions of N76R1/A196R1 in the BR and O states, respectively. The distance distribution in O state was extracted from those in the illuminated samples by subtracting a scaled amount of distribution in the non-illuminated samples according to the occupancy of O in Table 1.The vertical (normalized population) axes of the distributions are arbitrary and selected for convenience of display.