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. Author manuscript; available in PMC: 2008 Feb 23.
Published in final edited form as: J Mol Biol. 2006 Dec 12;366(3):790–805. doi: 10.1016/j.jmb.2006.12.017

Table 1.

Characteristic absorption properties of bacteriorhodopsin mutants before and after spin labeling. All mutants contained the E204Q residue change as background. The occupancy of O is shown for the spin labeled mutants under continuous illumination in two different buffer systems

Location of spin label Abs.
max.(nm)
before
labeling		 Abs.
max.(nm)
after
labeling		 O
occupancy at
pD 6a
%		 O
occupancy at
pH 9b
%
--- 568 568 50 N. D.
L61R1 562 559 21 7
L62R1 567 566 32 13
Q75R1 568 567 50 19
N76R1 566 564 40 8
K129R1 567 566 40 7
V130R1 566 565 36 12
G195R1 568 567 30 8
A196R1 568 567 53 28
N76R1/A196R1 568 564 44 N. D.
K129R1/A196R1 567 566 28 N. D.
L61R1/K129R1 560 552 18 N. D.
N78R1 564 460-570c <5 N. D.
T128R1 566 550 <10 N. D.
Y131R1 564 555 <10 N. D.
G192R1 564 490-570c <5 N. D.
a

buffer: 10mM Pi/Na, 100mM NaCl, pD=6.0 in D2O

b

buffer: 10mM Pi/Na, 100mM NaCl, pH=9.0 in H2O

c

broad peak

N.D.: Not Determined