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TUNEL-stained sections of vein grafts of apoE+/+ (b and d) and apoE−/− (a, c, and e) mice. Vein grafts were harvested 4 (b and c) and 8 (a, d, and e) weeks after surgery, fixed in 4% phosphate-buffered formaldehyde, pH 7.2, embedded in paraffin, sectioned, and stained with TUNEL as described in Materials and Methods. a: Negative control. Open arrows indicate the surface of the intima; filled arrows indicate typical examples of TUNEL-positive cells in lesions.
