Figure 6.

Expression of both PECAM and ICAM-1 is up-regulated on vascular endothelium in early brain abscess lesions. Adult Lewis rats were implanted with S. aureus-containing or sterile beads. Brain sections from animals perfusion-fixed with 4% paraformaldehyde/0.1 mol/L phosphate buffer were stained with antibodies specific for PECAM or ICAM-1 or an isotype-matched control antibody for immunohistochemical analysis. Images shown represent tissues collected at 48 hours after implantation of S. aureus or sterile beads. Arrows delineate vascular endothelium expressing PECAM or ICAM-1. Original magnification, ×100.