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. 2000 Aug;157(2):449–461. doi: 10.1016/S0002-9440(10)64557-9

Figure 3.

Figure 3.

IP/Western blot analysis of Bim expression in transformed mouse (A) and human (C) cell lines. Bim proteins were immunoprecipitated from lysates of 10 7 cells with anti-Bim mAb 5E5 or 14A8. Western blotting was performed with the same antibodies, followed by biotinylated mouse anti-rat IgG2b antibodies and streptavidin-HRP (5E5) or with HRP-conjugated mouse anti-rat IgG2a antibodies (14A8) and ECL detection. Anti-Bim mAbs 5E5 and 14A8 immunoprecipitate BimL and BimL plus BimEL, respectively, only from lysates of spleen and thymus from normal but not those from Bim-deficient mice (B).