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. 2000 Aug;157(2):537–548. doi: 10.1016/s0002-9440(10)64564-6

Figure 1.

Figure 1.

Oligonucleotides used in PCR reactions and in the construction of DNA templates for GGT RNA standard synthesis. A: Sequence of the oligonucleotides. Positions of the oligonucleotides in the different GGT mRNA sequences are numbered 5′ to 3′ from the A (+1) in the initiation codon. Standard and quantitative amplifications of GGT transcripts I–V were performed with specific sets of primers A and B. B: Schematic representation of the construction of GGT DNA templates used to transcribe mRNA I–IV and mRNA V standards. Oligomers A (extended with T7 sequence), B, C (extended with tail 2), and D (extended with tail 1), used to construct DNA templates by directed in vitro mutagenesis, are mapped on diagrams of the 5′ region of GGT mRNAs I–IV and mRNA V. The open boxes correspond to the beginning of the reading frame; the thick lanes to the 5′ untranslated sequence from −1 to −144, common to all GGT transcripts; and the thin lanes stand for specific regions at each 5′ mRNA end. The NH-22 and SN-4 probes are mapped on the diagram.