Figure 2.

Southern blot analysis of GGT PCR products. Total RNA from 18-day-old liver embryos (lanes 1, 2, 4, 5: 400 ng; lane 3: 5 ng) was reverse transcribed and amplified, using the five different sets of oligomers A and B (Figure 1) ▶ specific for GGT mRNAs I, II, III, IV, or V (lanes 1–5). Amplified products were separated on an agarose gel, blotted, and analyzed by hybridization to two internal oligomers (NH-22, specific for mRNAs I–IV, and SN-4, specific for mRNA V). The sizes reported on the autoradiogram were deduced from a DNA ladder run on the same gel. The blot was scanned on STORM 840 Phosphor Screen (Molecular Dynamics).