Figure 3.

Absent or markedly decreased constitutive SHP-1 mRNA expression and PMA-induced SHP-1 protein expression in malignant T cells. T cell lines from cutaneous T cell lymphoma (PB-1, 2A, 2B, and Sez-4) and HTLV-I-positive adult-type T cell leukemia/lymphoma (C91PL, ATL-2, HUT102B, and C10MJ2), and control PBMC from healthy donors and HS445 cells were examined for expression of the SHP-1 mRNA (A). Detection of actin mRNA (B) served as a control of the quality and quantity of mRNA in the samples. The T cell lines were stimulated with phorbol ester (PMA) and examined for expression of SHP-1 protein (C). (−)*, denotes the control, PMA-unstimulated cells