Table 2.

Primers, PCR, and Gel Conditions Used for SSCP Analysis of the p57^KIP2 Gene

	Primers forward (fw)-reverse (rv)	PCR conditions	Gel conditions (acrylamide:bis)
Frag 1	fw 5′GTCCACCACCGGACAGCCAG3′	1.0 mmol/L MgCl, 10% DMSO	10% 29:1 22°C
	rv 5′CGACAAGACGCTCCATCGTG3′	35 cycles 94°C× 35 seconds/62°C× 40 seconds/72°C× 40 seconds	10% 79:1 4°C
Frag 2	fw 5′CGTCCCTCCGCAGCACATCC3′	1.0 mmol/L MgCl, 10% DMSO	10% 29:1 4°C
	rv 5′CCTGCACCGTCTCGCGGTAG3′	35 cycles 94°C× 35 seconds/60°C× 40 seconds/72°C× 40 seconds	10% 29:1 10% glycerol 22°C
Frag 3	fw 5′TGGACCGAAGTGGACAGCGA3′	1.0 mmol/L MgCl, 10% DMSO	10% 29:1 22°C
	rv 5′GGGGCCAGGACCGCGACC3′	35 cycles 94°C× 35 seconds/62°C× 40 seconds/72°C× 40 seconds	10% 79:1 4°C
Frag 4	fw 5′CGGAATTCCGGAGCAGCTGCCTAGTGTC3′	1.0 mmol/L MgCl, 10% DMSO, 7-deaza dGTP	10% 49:1 4°C
	rv 5′CGTCCCGAAATCCCCGAGTGC3′	40 cycles 94°C× 35 seconds/63°C× 30 seconds/72°C× 30 seconds
Frag 5	fw 5′CCGAGCAGGGCGCGAACCAG3′	1.0 mmol/L MgCl, 10% DMSO	10% 29:1 22°C
	rv 5′CACGGGAGGAGGCGGGAACC3′	35 cycles 94°C× 35 seconds/62°C× 40 seconds/72°C× 40 seconds	10% 29:1 10% glycerol 4°C
Frag 6	fw 5′TGTCGCCCGCAGATTTCTTC3′	1.0 mmol/L MgCl, 10% DMSO	10% 29:1 10% glycerol 22°C
	rv 5′CCCAGGTGCGCTGTACTCAC3′	35 cycles 94°C× 35 seconds/60°C× 40 seconds/72°C× 40 seconds	10% 29:1 10% glycerol 4°C

Abbreviations: Frag, fragment; DMSO, dimethyl sulfoxide; MgCl, magnesium chloride (MgCl₂).