Table 4.

Imprint Status of KIP2, H19, and IGF2 in Hepatoblastomas and HB Cell Lines

RNA	Informativity KIP2#	Imprint KIP2	Informativity H19 RsaI	Imprint H19 RsaI	Informativity IGF2	Imprint IGF2
D269	No	—	No	—	No	—
D272	No	—	No	—	No	—
D266	No	—	No	—	Yes	MOI
D263	Yes	MOI	No	—	No	—
D166	Yes	LOH	No	—	No	—
D23II	—	—	—	—	No	—
D195	No	—	Yes	MOI	No	—
D197	Yes	MOI	Yes	MOI	No	—
DZ18	—	—	—	—	No	—
M12	No	—	No	—	No	—
M14	Yes	MOI*	No	—	Yes	LOI
D401	No	—	No	—	No	—
D104	Yes	LOH	No	—	No	—
D199	No	—	No	—	Yes	MOI
D497	No	—	No	—	No	—
D268	Yes	LOH	No	—	No	—
D161	Yes	MOI*	No	—	Yes	LOI
DZ29II	No	—	Yes	MOI	No	—
DZ20	Yes	MOI*	No	—	No	—
DZ25II	Yes	LOH	No	—	No	—
D175	Yes	MOI	Yes	MOI	No	—
D204	No	—	No	—	No	—
D319	Yes	MOI	No	—	No	—
D399II	Yes	MOI	No	—	No	—
DZ27II CL HepT1	Yes+	LOH	No	—	No	—
D501 CL HepT3	—	—	No	—	No	—
D339 CL HUH6	Yes	MOI	Yes	MOI	Yes	LOI

Status of imprinting for p57^KIP2, H19, and IGF2. Informativity of this polymorphism was assessed by the amplification of genomic DNA from normal tissue if available. Abbreviations: MOI, maintenance of imprint; LOI, loss of imprint; MOI*, leaky expression from the second allele visible on silver-stained polyacrylamide gel electrophoresis gel; +, genomic DNA of the original tumor’s adjacent original liver tissue has been used for comparison; LOH, loss of heterozygosity (monoallelic expression).