Figure 3.

OSM stimulates anchorage-independent growth. Single cell suspensions of mouse synovial fibroblasts were suspended in 0.4% agarose in Dulbecco’s modified minimal essential medium containing 20% fetal bovine serum and the indicated cytokines at a concentration of 10 ng/ml. All treatments were completed in triplicate. After 14 to 17 days, the resultant cell colonies were optically graded for size as being 0 to 20 μm in diameter (single cells or small groups, white bars), of >20 μm (true colonies, black bars). Data shown is representative of two separate experiments.