Figure 4.

AdlacZ infection of synovial cells in vitro and in vivo. A: Primary mouse synovial fibroblasts were untreated or infected with AdmCMVlacZ or AdmuOSM at multiplicity of infection of 10 and 5. Fibroblasts were stained for lacZ expression with X-gal. The blue staining demonstrates expression of lacZ. B: Mice were treated with intra-articular AdlacZ (5 × 10 7 pfu) and sacrificed 24 hours later. Joints were removed, fixed, decalcified, and stained for lacZ expression. Blue staining was consistently observed in synovial lining cells.