Figure 2.

Southern blot hybridization of a cDNA expression array with ³²P-labeled cDNA probes prepared from mRNA isolated from SFK and SFH cells. A set of filters containing 597 arrayed mouse cDNA clones of various functional classes, immobilized as a spot of duplicate dots on a nylon membrane, were hybridized with ³²P-labeled cDNA probes prepared from poly A⁺ RNA that was isolated from bFGF-treated or untreated SFK and SFH cells maintained for 4 months in vitro: SFH-C (a), SFK-C (b), SFH-B (c), or SFK-B (d) cells. The spots indicated by arrows represent the clones of (1) the receptor tyrosine kinase substrate Eps8, (2) cyclin D1, (3) CD44, (4) phosphatidylinositol 3-kinase (PI3K) p85, (5) insulin-like growth factor (IGF-I), (6) serine protease inhibitor (Spi-2.2), and (7) β-actin.