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. 2000 Jul;157(1):249–255. doi: 10.1016/s0002-9440(10)64535-x

Figure 5.

Figure 5.

Inhibition of stimulation of FGF7 secretion by CM with inhibitors of IL-1 α action. A: Primary cultures of prostatic stromal cells were plated in RPMI 1640 with insulin, transferrin, and selenium and supplemented with a 1:10 dilution of conditioned medium (CM) prepared from two separate primary epithelial cultures (CM1 and CM2); diluted CMs preincubated with excess anti-Il-1α neutralizing antibody; diluted CM1 preincubated with anti-VEGF (vascular endothelial cell growth factor) antibody; Il-1α at 6 pg/ml; or Il-1α at 6 pg/ml preincubated with anti-Il-1α neutralizing antibody or were not supplemented (control). FGF7 concentration was determined by ELISA after 48 hours of treatment. B: Primary cultures of prostatic stromal cells were plated in RPMI 1640 with insulin, transferrin, and selenium and supplemented with a 1:10 dilution of conditioned medium (CM) prepared from two separate primary epithelial cultures (CM1 and CM2); diluted CMs after cells were pretreated with recombinant IL-1Ra or Il-1Ra only, or were not supplemented (control). FGF7 concentration was determined by ELISA after 48 hours of treatment.