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DNA fragmentation of PMNs cultured for 18 hours. Lane 1: markers; lane 2: unprimed neutrophils alone; lane 3: primed PMNs incubated with MPO-ANCA; lane 4: primed PMNs incubated with N IgG; lane 5: primed PMNs incubated with PR3-ANCA; lane 6: primed PMNs alone.
