Figure 1.

Adhesion molecule expression by breast carcinoma cells. Cell lines were treated with M199/A alone (time, 0) or M199/A containing 20 ng/ml TNF-α for 4 or 24 hours (time, 4, 24). A: After the incubation, cells were lysed in TRIzol and total RNA was isolated. RT-PCR was performed as described in Methods. B: Alternatively, cells were lysed in Triton X-100 containing buffer and the soluble proteins were separated on SDS-PAGE. Western blotting was performed using 2 μg/ml of the specified mAbs directed against P-selectin (G1), E-selectin (ES-1), ICAM-1 (BBA 4), or VCAM-1 (1.G11B1). Experiments were performed at least three times with equivalent data. Representative data are shown.