Figure 4.

Eosinophil adhesion to MDA-MB-435S and BT-20 cells requires α4-integrins. MDA-MB-435S cells were treated with buffer alone (A) or TNF-α (B) for 24 hours as described in Figure 1 ▶ . Alternatively, BT-20 cells were treated TNF-α for 24 hours as described in Figure 1 ▶ (C). MDA-MB-435S (A and B) or BT-20 cells (C) were pretreated for 15 minutes at 37°C with 5 μg/ml of the specified mAbs directed against ICAM-1 (BBA4) or VCAM-1 (1.G11B1). Alternatively, eosinophils were preincubated with α4-integrin mAb (H2/1) or β2-integrin mAb (7E4) for 15 minutes before addition to the cell monolayers. Eosinophil adhesion was determined in the continued presence of the specified mAbs as described in Methods. Data are mean ± SEM of at least three experiments. * P < 0.05 as compared to control.