Figure 6.

Breast carcinoma cells release eosinophil chemoattractant(s). Supernatants from MDA-MB-435S or BT-20 cells were collected as described in Figure 5 ▶ . Freshly isolated eosinophils (4 × 10⁶/ml) were added to the upper chamber of a 3-μm pore transwell dish coated with 25 μg/ml of fibronectin. Supernatants were placed in the lower chamber at a 1:10 dilution. M199/A or M199/A with an equivalent amount of TNF-α was used as controls (control). Eosinophils that had migrated into the lower chamber after 90 minutes were counted as described in Methods. Experiments were performed three times with equivalent data. Data represent the mean ± range of a single experiment.