Figure 7.

MMP activity in different organs of mice after LPS-injection. Mice were killed 12 hours after i.p. LPS injection (20 μg) and organs were frozen in liquid nitrogen. Frozen organs were homogenized in lysis buffer and protein content determined as described in Materials and Methods. Unreduced samples were fractionated on 10% acrylamide gels containing 1 mg/ml gelatin. Subsequently, gelatin zymography was performed as described in Materials and Methods. Protein loading was 50 μg for brain, 20 μg for kidney, 6 μg for spleen, and 7 μg for liver. The constitutive gelatinolytic activity in the brain is not easily visible in this reproduction.