Table 3.
Microsatellite Alterations in Tumors and p53 Immunohistochemistry
| Study No. | Staging (acc. to WHO 1978) | p16* | DPC4* | p53* | p53 protein† |
|---|---|---|---|---|---|
| PC 1 | pT1a,pN0,pMX,R0,G2 | HD(3/7) | LOH(5/5) | LOH(5/7) | (+)/(+++) |
| PC 2 | pT1a,pNX,pMX,R0,G3 | HD(2/3), LOH(1/3) | n(2/2) | n(2/2) | (−) |
| PC 3 | pT3,pN1,pMX,G3 | HD(2/3) | HD(2/3) | LOH(1/3) | n.d. |
| PC 4 | pT1b,pN0,pMX,R0,G3 | HD(4/4) | LOH(5/5) | LOH(2/5) | (−) |
| PC 5 | pT2,pN1,pMX,R1,G1 | LOH(2/4) | n(5/5) | n(5/5) | (+++) |
| PC 6 | pT1,pN0,pMX,R0,G1 | n.i. | LOH(2/4) | n(3/3) | n.d. |
| PC 7 | pT2,pN0,pMX,R0,G2 | LOH(3/3) | LOH(3/3) | LOH(2/2) | (++) |
| PC 8 | pT1b,pN1,pMX,R0,G2 | n.d. | HD(1/2), LOH(1/2) | LOH(2/2) | n.d. |
| PC 9 | pT2,pN1,pMX,R0,G2 | n.d. | LOH(2/4) | n(3/3) | n.d. |
| PC 10 | pT3,pN0,pMX,R1,G2 | n.i. | LOH(1/2) | LOH(1/2) | n.d. |
| PC 11 | pT2,pN1,pMX,R0,G3 | n(2/2) | LOH(3/5) | n.i. | (+++) |
| PC 12 | pT2,pN0,pMX,R0,G2 | n(2/2) | n(7/7) | LOH(4/7) | (−) |
| PC 13 | pT2,pN1,pMX,R1,G2 | LOH(1/2) | n(2/2) | LOH(1/1) | (+++) |
| PC 14 | pT2,pN1,pMX,G2 | LOH(1/1) | n.i. | LOH(3/4) | (−)/(+++) |
| PC 15 | pT2,pN1,pM0,R0,G3 | n.i. | n.i. | LOH(1/2) | (+++) |
| PC 16 | pT2,pN1,pMX,R0,G3 | LOH(1/2) | n.i. | LOH(3/3) | (++) |
| PC 17 | pT2,pN0,pMX,R0,G3 | HD(3/3) | LOH(1/3) | LOH(2/3) | (+)/(+++) |
| PC 18 | pT1,pN0,pMX,R0,G1 | HD(1/1) | n(2/2) | n(3/3) | (−) |
| PC 19 | pT2,pN1,pMX,R0,G2 | n.d. | HD(1/2) | LOH(1/2) | n.d. |
| PC 20 | pT2,pN1,pMX,R0,G3 | n(2/2) | n.i. | LOH(2/2) | (−)/(+++) |
| PC 21 | pT2,pN1,pMX,R0,G2 | n(3/3) | n(3/3) | LOH(1/3) | (++) |
| PC 22 | pT2,pN1,pMX,R0,G2 | HD(3/4), LOH(1/4) | HD(3/4), LOH(1/4) | LOH(4/4) | n.d. |
| PC 23 | pT2,pN1,pMX,R0,G2 | n(2/2) | LOH(2/3) | LOH(4/4) | (+++) |
| PC 24 | pT2,pN1,pMX,R0,G2 | HD(2/3), LOH(1/3) | LOH(2/3) | LOH(3/3) | n.d. |
| PC 25 | pT2,pN0,pMX,R0,G2 | n.d. | LOH(4/5) | LOH(3/5) | (+++) |
| PC 26 | pT2,pN1,pMX,R0,G3 | n(3/3) | LOH(2/4) | LOH(3/4) | (−)/(++) |
| PC 27 | pT1a,pN0,pMX,R0,G3 | n(2/2) | LOH(1/3) | LOH(1/3) | (−) |
| PC 28 | pT3,pN1,pMX,R1,G3 | n(2/2) | n(2/2) | n.i. | (+++) |
| PC 29 | pT2,pN1,pMX,R1,G2 | LOH(2/2) | n(2/2) | LOH(2/2) | (++) |
*For tumor suppressor genes, numbers in parentheses show the number of LOHs detected/number of tumor foci microdissected. n, normal; LOH, loss of heterozygosity; HD, homozygous deletion; n.d., not done; n.i., not informative.
†Immunohistochemical staining of p53 protein was classified from no positivity (−), low labeling (+/++), and high labeling (+++).