Figure 4.

A: Double-staining FACS analysis of the phenotype of decidual cells (nonadherent fraction after overnight culture). Approximately 4.5% of the cells are positive for CD83 (upper left) and these co-stain with HLA-DR, CD40, CD45 and, at a very low level, with CD14, but not with CD56. A population of CD83⁻ cells weakly positive for HLA-DR, CD40, and CD14 may represent macrophages, the CD56⁺ cells (lower right) represent the LGLs typical for human decidua. B: Flow cytometry of immunomagnetically-enriched decidual DC demonstrated 79.8% of the cells to be CD45⁺, 32.5% to be CD83⁺, and all CD83⁺ cells to be highly positive for HLA-DR. A small population of CD83⁺ cells co-stains with CD14 (3.5%) or CD1a (6.3%). A population of CD83⁻ but weak HLA-DR⁺ cells (12.7%) may represent immature DC. The main population of the CD83⁻ cells are CD3⁺ T cells (23.3% of all cells).