Table 1.
Antibodies Used in This Study
| Antigen | Clone | Species/isotype | Major specificities | Supplier |
|---|---|---|---|---|
| CD1a | HI149 | Mouse, IgG1 | Langerhans cells, cortical thymocytes, subsets of DC | Pharmingen |
| CD3 | UCHT1 | Mouse, IgG1 | All T cells | Dianova |
| CD14 | UCHM1 | Mouse, IgG2a | Monocytes, macrophages, granulocytes | Serotec |
| CD40 | B-B20 | Mouse, IgG1κ | Lymphoid dendritic cells, mature peripheral B-cells | Dianova |
| CD45 | MEM28 | Mouse, IgG1 | All leukocytes | Dianova |
| CD56 | NCAM16.2 | Mouse, IgG2b | NK-cells, T cell subsets | Becton Dickinson |
| CD83 | HB15a | Mouse, IgG2b | Mature DC | Immunotech |
| HLA-DR | DDII | Mouse, IgG1 | Monocytes, macrophages, DC, B lymphocytes | Dianova |
| Cytokeratin | KL1 | Mouse, IgG1 | CK 2,5,6,8,10,11,14,15,18,19: epithelial cells, trophoblasts | Immunotech |